HEAT AND MASS EXCHANGE IN PROTEIN PRODUCTION DEVICES BASED ON HYDROGEN OXIDIZING BACTERIA
Abstract
Global economic development trends urgently require the transition of agricultural food production to industrial scale.
Numerous patents recently obtained confirm that many countries are interested in protein production processes, particularly those based on hydrogen-oxidizing bacteria.
Research into this process is currently in the laboratory experimentation and preliminary design stages for pilot plants.
This paper examines the current state of development in protein production using hydrogen-oxidizing bacteria. To identify deficiencies in the biomass cultivation process, a pilot plant was created and a series of studies were conducted. The results were compared with existing data.
Based on the experimental results, the key principles of the biomass production process were clarified, and the reliability of individual components of the laboratory setup was verified.
A comprehensive analysis of the resulting biomass, conducted in I. Mechnikov Institute of Microbiology and Immunology (Kharkiv, Ukraine) established that, during continuous cultivation, protein synthesis by the Alkaligenes eutropha Z1 bacterium is stable, accounting for 72-74 % of the resulting biomass, consistent with published data. The content of lipid, carbohydrate components, and nucleic acids is within normal limits. The presence of other species in the biomass does not exceed permissible levels.
Thus, it has been proven that the individual components and the entire system operate reliably.
Based on the results of experimental studies of the process, it was recommended to change the equipment design of the stand – to dissolve hydrogen in the cultural liquid before feeding it to the fermenter, and to use film devices as fermenters with stirrers.
A calculation sequence for heat and mass transfer in a film fermenter was developed.
It has been shown that the conversion of protein production from agricultural methods to industrial ones will yield significant economic benefits and improve the environment by reducing harmful emissions into the atmosphere.
References
2. Pat. WO 2024/253609 A1. Sustainable waste management system of bio-circular technology involving a microbial consortium. Int. Cl.: B09B3/00, C12P1/04. Inventor; and Applicant: Basal, Ahmet (TR). Agent: Kordinat inovasyon ve fikri mulkiyet yonetimi Ltd. Stl; 34340 Besiktas/Istanbul (TR). Appl.: PCT/TR2023/050690. Filed: 18.07.2023. Publ. Date: 12.12.2024.
3. Pat. US 10,696,941 B2. Method and apparatus for growing microbial cultures that require gaseous electron donors, electron acceptors, carbon sources, or other nutrients. Int. Cl.: C12M3/00, C12M1/20. U.S. Cl.: C12N1/20, C12M41/46, C12M41/48. Inventors: Peter Dalla-Betta, Hayward, CA (US); John S. Reed, Berkeley, CA (US). Assignee: Kiverdi, Inc., Berkeley, CA (US). Appl. No.: 14/839,074. Filed: Aug. 28, 2015. Publ. Date: Jun. 30, 2020.
4. GB 2594454 A. Method for producing biomass using hydrogen-oxidizing bacteria. Int. Cl.: C12N1/20, A23K10/10, B01D53/84, C12P1/04, C12Q3/00, B01D2251/95. Inventors: Patrick William Mansfield Robert, Pander Bart, Krabben Preben, Nicholas Rowe Peter. Assignee: Deep Branch Biotechnology Ltd.; Crown Way, Cardiff CF14 3UZ, (UK). Appl.: No.: 2006071.1. Filed: 24.04.2020. Publ. Date: 03.11.2021
5. Pat. EP 4473125 A1. Process for biotechnological production of a bioproduct. Int. Cl.: C12P7/6436, C12M23/58, C12M43/00. Inventors: Philipp Arbter, Tyll Utesch, An-Ping Zeng. Current Assignee: Colipi GmbH; Hamburg (DE). Appl.: EP22709595.7A. Filed: 01.02.2022. Publ. Date: 11.12.2024.
6. Messerle V.E., Ustimenko A.B. [Thirty-first International Technical Conference on Coal Utilization and Fuel Systems] / Teploenergetika [Thermal power engineering]. 2007. No. 3. - P. 71-76. (in rus.)
7. Volova T.G., Terskov I.A., Sidko F.Ya. [Microbiological synthesis on hydrogen]. - Nauka, 1985. - 116 pp. (in rus.)
8. Pat. AU 2021277653 B2. Microorganisms and artificial ecosystems for the production of protein, food, and useful co-products from C1 substrates. Int. Cl. C12P1/04, C12P5/00, C12M1/04, C12P7/64, C25B1/04. Inventors: Reed, John S.; Geller, Jil; Hande, Sonali. Kiverdi, Inc.; Agent/Attorney: Arcadia Intellectual Property, Level 40 140 William Str., Melbourne, VIC, 3000 (AU). Appl. No: 2021277653. Filed: 30.11.2021. Publ. Date: 21.12.23.
9. Birykov V.V., Каnterе V.М. [Optimization of batch processes of microbiological synthesis]. - Nauka, 1985. - 296 с. (in rus.)
10. GTM 26-01-71-75 [(Guiding technical material). Evaporators with flowing film. Methods of thermal and hydromechanical calculations]. - M.: [Ministry of Chemistry. and petroleum engineering], 1975, - 43 pp. Access mode: https://www.twirpx.com/file/3494809/ (in rus.).
11. Troshen'kin B.A. [Circulating and film evaporators and hydrogen reactors]. - Kyiv: Naukova Dumka, 1985. - 176 pp. Access mode: https://www.twirpx.com/file/3495851/ (in rus.).
12. Chybin I.А., Маslov А.М. [Handbook of thermophysical characteristics of food products and semi-finished products] // Pishchevaya promishlennost [Food industry]. 1970. - 184 pp. (in rus.).
13. Мigai V.Е., Firsova E.V. [Heat transfer and hydraulic resistance of tube bundles]. - Nauka. 1986. - 195 pp. (in rus.)
14. Pat. UA112569. [Method of underground gasification of coal and composition for its implementation]. Int. Cl. E21B 43/295, C10G 1/00. Inventors: Troshenkin B.O., Yanko S.V., Troshenkin V.B., Khomyak K.M.; Owner: A. Pidgorny Inst. of Mechanical Eng. Problems of NAS of Ukraine. - No. a 201409035; Appl.: 11.08.2014; Publ. 29.06.2016,
Bull. No. 18. - 10 p. (in ukr.).
15. Мishchenko А.I. [Use of hydrogen for automobile engines]. - Kyiv: Nauka, 1984. - 143 pp. (in rus.)
16. Falkovski, P.G. Life’s Engines: How Microbes Made Earth Habitable. (Pop Science). Princeton and Oxford: Princeton University Press. 2015. ISBN 978-0691155371.
17. Levchyk I.V. [Scientific and methodological foundations of improving the technochemical control of raw materials and finished products of oil and fat production]. Author's abstract …diss. … doct. of techn. sciences. - Kharkiv: NTU kHPI. 2018. - 42 pp. (in ukr.).
Abstract views: 88 PDF Downloads: 61
If the article is accepted for publication in the journal «Thermophysics and Thermal Power Engineering» the author must sign an agreementon transfer of copyright. The agreement is sent to the postal (original) or e-mail address (scanned copy) of the journal editions.
